The time course of acclimation of critical thermal maxima is modulated by the magnitude of temperature change and thermal daily fluctuations

Plasticity in the critical thermal maximum (CTmax) helps ectotherms survive in variable thermal conditions. Yet, little is known about the environmental mechanisms modulating its time course. We used the larvae of three neotropical anurans (Boana platanera, Engystomops pustulosus and Rhinella horribilis) to test whether the magni-tude of temperature changes and the existence of fluctuations in the thermal environment affected both the amount of change in CTmax and its acclimation rate (i.e., its time course). For that, we transferred tadpoles from a pre-treatment temperature (23 degrees C, constant) to two different water temperatures: mean (28 degrees C) and hot (33 degrees C), crossed with constant and daily fluctuating thermal regimes, and recorded CTmaxvalues, daily during six days. We modeled changes in CTmax as an asymptotic function of time, temperature, and the daily thermal fluctuation. The fitted function provided the asymptotic CTmax value (CTmax & INFIN;) and CTmax acclimation rate (k). Tadpoles achieved their CTmax & INFIN; between one and three days. Transferring tadpoles to the hot treatment generated higher CTmax & INFIN; at earlier times, inducing faster acclimation rates in tadpoles. In contrast, thermal fluctuations equally led to higher CTmax & INFIN; values but tadpoles required longer times to achieve CTmax & INFIN; (i.e., slower acclimation rates). These thermal treatments interacted differently with the studied species. In general, the thermal generalist Rhinella horribilis showed the most plastic acclimation rates whereas the ephemeral-pond breeder Engystomops pustulosus, more exposed to heat peaks during larval development, showed less plastic (i.e., canalized) accli-mation rates. Further comparative studies of the time course of CTmax acclimation should help to disentangle the complex interplay between the thermal environment and species ecology, to understand how tadpoles acclimate to heat stress.

Automated summary

The study found that the thermal environment affects the changes in the critical thermal maximum (CTmax) and its acclimation rate in neotropical anuran larvae. The hot treatment resulted in higher CTmax values at earlier times, leading to faster acclimation rates, while thermal fluctuations led to higher CTmax values but slower acclimation rates. These effects varied across different species.