Interfacial compositions of fat globules modulate structural characteristics and lipolysis of its model emulsions during in-vitro digestion

BACKGROUND: This study investigated whether milk fat globule membrane as an emulsifier could make fat easier for infants to digest. An emulsion was formed using the membrane material, where anhydrous milk fat was used as the core material, milk fat globule membrane polar lipid (MPL) as the emulsifier, and soybean phospholipid (PL) and milk protein concentrate (MPC) incorporated as control emulsifiers. Structural characterization, glyceride composition, and fatty acid release from emulsions by in vitro digestion were investigated. RESULTS: The average particle size at the end of intestinal digestion was in the order MPL < PL < MPC, with diameters of 3.41 +/- 0.51 mu m, 3.53 +/- 0.47 mu m, and 10.46 +/- 2.33 mu m respectively. Meanwhile, laser scanning confocal microscopy results also illustrated that MPL could reduce the degree of aggregation during digestion. The lipolysis degree of MPL emulsion was higher than that of PL and MPC emulsions. MPL not only released higher levels of long-chain fatty acids, such as C18:1, C18:2, C18:3, which are of great significance for infant growth and development, but also released increased levels of C20:4 (arachidonic acid) and C22:6 (docosahexaenoic acid) than PL and MPC emulsions did. CONCLUSION: Fat droplets enveloped by milk fat globule MPLs were easier to digest and are therefore more suitable for infant formula . (c) 2023 Society of Chemical Industry.

Automated summary

This study investigated the use of milk fat globule membrane as an emulsifier to improve the digestibility of fat in infant formula. Results showed that the membrane reduced aggregation during digestion and released higher levels of important long-chain fatty acids compared to other emulsifiers. This makes fat droplets enveloped by milk fat globule membranes more suitable for infant formula.