Bioethanol production from sorghum grain with Zymomonas mobilis: increasing the yield and quality of raw distillates

BACKGROUNDThe present study aimed to demonstrate the superiority of bioethanol yield and its quality from sorghum using the granular starch degrading enzyme Stargen (TM) 002 over simultaneous saccharification and fermentation, and separate hydrolysis and fermentation using Zymomonas mobilis CCM 3881 and Ethanol Red (R) yeast. RESULTSBacteria were found to produce ethanol at higher yield than the yeast in all fermentations. The highest ethanol yield was obtained with Z. mobilis during 48 h of simultaneous saccharification and fermentation (83.85% theoretical yield) and fermentation with Stargen (TM) 002 (81.27% theoretical yield). Pre-liquefaction in fermentation with Stargen (TM) 002 did not improve ethanol yields for both Z. mobilis and Saccharomyces cerevisiae. Chromatographic analysis showed twice less total volatile compounds in distillates obtained after bacterial (3.29-5.54 g L-1) than after yeast (7.84-9.75 g L-1) fermentations. Distillates obtained after bacterial fermentation were characterized by high level of aldehydes (up to 65% of total volatiles) and distillates obtained after yeast fermentation of higher alcohols (up to 95% of total volatiles). The process of fermentation using granular starch hydrolyzing enzyme cocktail Stargen (TM) 002 resulted in low amounts of all volatile compounds in distillates obtained after bacterial fermentation, but the highest amounts in distillates obtained after yeast fermentation. CONCLUSIONThe present study emphasizes the great potential of bioethanol production from sorghum with Z. mobilis using granular starch hydrolyzing enzyme Stargen (TM) 002, which leads to reduced water and energy consumption, especially when energy sources are strongly related to global climate change. (c) 2023 Society of Chemical Industry.

Automated summary

The present study demonstrates the superiority of bioethanol production from sorghum using the granular starch degrading enzyme Stargen (TM) 002 compared to simultaneous saccharification and fermentation and separate hydrolysis and fermentation. Bacteria produce ethanol at a higher yield than yeast in all fermentations. Using Z. mobilis for simultaneous saccharification and fermentation and Stargen (TM) 002 for fermentation resulted in the highest ethanol yield.