期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 145, 期 23, 页码 12576-12585出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.3c01326
关键词
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Inspired by plant cell walls, a method was developed to prepare membranized coacervates as protocell models by coating membraneless liquid-like microdroplets with rigid polysaccharides. The membranization enhanced stability, prevented aggregation, facilitated selective biomolecule sequestration, and allowed chemical exchange across the membrane. The polysaccharide wall surrounding the protocells acted as a stimuli-responsive structural barrier for enzyme-triggered membrane lysis and internalization of bacteria, offering a means to mimic cell behavior.
The design of compartmentalized colloidsthat exhibit biomimeticproperties is providing model systems for developing synthetic cell-likeentities (protocells). Inspired by the cell walls in plant cells,we developed a method to prepare membranized coacervates as protocellmodels by coating membraneless liquid-like microdroplets with a protectivelayer of rigid polysaccharides. Membranization not only endowed colloidalstability and prevented aggregation and coalescence but also facilitatedselective biomolecule sequestration and chemical exchange across themembrane. The polysaccharide wall surrounding coacervate protocellsacted as a stimuli-responsive structural barrier that enabled enzyme-triggeredmembrane lysis to initiate internalization and killing of Escherichia coli. The membranized coacervates werecapable of spatial organization into structured tissue-like protocellassemblages, offering a means to mimic metabolism and cell-to-cellcommunication. We envision that surface engineering of protocellsas developed in this work generates a platform for constructing advancedsynthetic cell mimetics and sophisticated cell-like behaviors.
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