Insights of Life Molecules' Dynamic Distribution in Live Cells via Sequence-Structure Bispecific Fluorescent RNA

Life molecules' distributions in live systemsconstructthe complex dynamic reaction networks, whereas it is still challengingto demonstrate the dynamic distributions of biomolecules in live systems.Herein, we proposed a dynamic analysis strategy via sequence-structure bispecific RNA with state-adjustable moleculesto monitor the dynamic concentration and spatiotemporal localizationof these biomolecules in live cells based on the new insight of fluorescentRNA (FLRNA) interactions and their mechanism of fluorescence enhancement.Typically, computer-based nucleic acid-molecular docking simulationand molecular theoretical calculation have been proposed to providea simple and straightforward method for guiding the custom-designof FLRNA. Impressively, a novel FLRNA with sequence and structurebispecific RNA named as a structure-switching aptamer (SSA) was introducedto monitor the real-time concentration and spatiotemporal localizationof biomolecules, contributing to a deeper insight of the dynamic monitoringand visualization of biomolecules in live systems.

Automated summary

The distributions of life molecules in live systems form complex dynamic reaction networks, and it is challenging to demonstrate their dynamic distributions in live systems. We proposed a dynamic analysis strategy using sequence-structure bispecific RNA with state-adjustable molecules to monitor the dynamic concentration and spatiotemporal localization of biomolecules in live cells, based on the insight of fluorescent RNA (FLRNA) interactions and their mechanism of fluorescence enhancement. A structure-switching aptamer (SSA), a novel FLRNA with sequence and structure bispecificity, was introduced to monitor the real-time concentration and spatiotemporal localization of biomolecules, providing a deeper understanding of the dynamic monitoring and visualization of biomolecules in live systems.