Weak transgenerational effects of ancestral nitrogen and phosphorus availabilities on offspring phenotypes in Arabidopsis thaliana

Nutrient availability significantly regulates plant growth and metabolic functions, but whether and how the long-term exposure of ancestral plants to contrasting nutrient environments influences offspring phenotypic performance (i.e., transgenerational plasticity) remain poorly addressed. Here we conducted experimental manipulations using Arabidopsis thaliana with the ancestral plants grown in different nitrogen (N) and phosphorus (P) availabilities over eleven consecutive generations, and then examined the offspring phenotypic performance under the interactive effects of current and ancestral nutrient environments. We found that current rather than ancestral nutrient environments dominantly explained the variations in offspring plant traits (i.e., flowering time, aboveground biomass and biomass allocation fractions), suggesting the relatively weak transgenerational effects of ancestral N and P availabilities on offspring phenotypes. In contrast, increasing N and P availabilities in the offspring generation remarkably shortened the flowering time, increased the aboveground biomass, and altered biomass allocation fractions differentially among organs. Despite the overall weak transgenerational phenotypic plasticity, under the low nutrient environment, the offspring of ancestral plants from the low nutrient environment had a significantly higher fruit mass fraction than those from the suitable nutrient environment. Taken together, our findings suggest that A. thaliana exhibits a much stronger within- than trans-generational trait plasticity under contrasting nutrient availabilities, and may provide important insights into the understanding of plant adaptation and evolutionary processes under changing nutrient environments.

Automated summary

Nutrient availability significantly affects plant growth and metabolic functions. This study investigates if and how long-term exposure of ancestral plants to different nutrient environments influences offspring phenotypic performance. Results show that current nutrient environments have a stronger impact on offspring traits compared to ancestral nutrient environments. However, increasing nutrient availability in the offspring generation leads to changes in flowering time, biomass, and biomass allocation fractions among organs. Overall, this study highlights the importance of within-generational trait plasticity in plant adaptation and evolutionary processes under changing nutrient environments.