Transcriptome analysis of the effects of polarized photobiomodulation on human dermal fibroblasts

Background: Photobiomodulation (PBM), the therapeutic use of light, is used to treat a myriad of conditions, including the management of acute and chronic wounds. Despite the presence of clinical evidence surrounding PBM, the fundamental mechanisms underpinning its efficacy remain unclear. There are several properties of light that can be altered in the application of PBM, of these, polarization-the filtering of light into specified plane (s)-is an attractive variable to investigate. Aims: To evaluate transcriptomic changes in human dermal fibroblasts in response to polarized PBM. Results: A total of 71 Differentially Expressed Genes (DEGs) are described. All DEGs were found in the polarized PBM group (P-PBM), relative to the control group (PC). Of the 71 DEGs, 10 genes were upregulated and 61 were downregulated. Most DEGs were either mitochondrial or extracellular matrix (ECM)-related. Gene Ontology (GO) analysis was then performed using the DEGs from the P-PBM vs. PC group. Within biological processes there were 95 terms found (p < 0.05); in the molecular function there were 18 terms found (p < 0.05); while in the cellular component there were 32 terms enriched (p < 0.05). A KEGG pathways analysis was performed for the DEGs found in the P-PBM vs. PC group. This revealed 21 significantly enriched pathways (p < 0.05). Finally, there were 24 significantly enriched reactome pathways when comparing the DEGs of the P-PBM vs. PC groups (p < 0.05). Discussion and conclusions: The P-PBM DEGs were almost always down regulated compared to the comparator groups. This may be explained by the P-PBM treatment conditions decreasing the amount of cellular stress, hence causing a decreased mitochondria and ECM protective response. Alternatively, it could point to an alternate mechanism, outside the mitochondria, by which PBM exerts its effects. Additionally, PBM appears to have a more widespread effect on the mitochondria than previously thought, opening up many new avenues of investigation in the process.

Automated summary

PBM is an effective therapy that uses light to treat various conditions, including wound management. This study aimed to evaluate the changes in gene expression in human dermal fibroblasts in response to polarized PBM. The results showed 71 differentially expressed genes (DEGs), with most DEGs being downregulated and involved in mitochondrial or extracellular matrix-related processes. The analysis also revealed enriched biological processes, molecular functions, and cellular components, as well as significantly enriched pathways. The findings suggest that P-PBM treatment reduces cellular stress and has a broader effect on mitochondria than previously thought.