Light Adaptation of Retinal Rod Bipolar Cells

The sensitivity of retinal cells is altered in background light to optimize the detection of contrast. For scotopic (rod) vision, substantial adaptation occurs in the first two cells, the rods and rod bipolar cells (RBCs), through sensitivity adjustments in rods and postsynaptic modulation of the transduction cascade in RBCs. To study the mechanisms mediating these compo-nents of adaptation, we made whole-cell, voltage-clamp recordings from retinal slices of mice from both sexes. Adaptation was assessed by fitting the Hill equation to response-intensity relationships with the parameters of half-maximal response (I1/2), Hill coefficient (n), and maximum response amplitude (Rmax). We show that rod sensitivity decreases in backgrounds according to the Weber-Fechner relation with an I1/2 of -50 Rp s21. The sensitivity of RBCs follows a near-identical func-tion, indicating that changes in RBC sensitivity in backgrounds bright enough to adapt the rods are mostly derived from the rods themselves. Backgrounds too dim to adapt the rods can however alter n, relieving a synaptic nonlinearity likely through entry of Ca2+ into the RBCs. There is also a surprising decrease of Rmax, indicating that a step in RBC synaptic transduction is desensitized or that the transduction channels became reluctant to open. This effect is greatly reduced after dialysis of BAPTA at a membrane potential of +50 mV to impede Ca2+ entry. Thus the effects of background illumination in RBCs are in part the result of processes intrinsic to the photoreceptors and in part derive from additional Ca2+-dependent processes at the first synapse of vision.

Automated summary

The sensitivity of retinal cells is altered in response to background light. The sensitivity adjustments in rods and postsynaptic modulation of the transduction cascade in rod bipolar cells are involved in this adaptation. The background illumination affects both rod and rod bipolar cell sensitivity.