DNA interactions, docking and in vitro cytotoxicity studies of [M(Hvalmea)2] complexes (M = CuII, CoIII)

Two new Schiff base mononuclear CuII and CoIII complexes were synthesized by the condensation of monoethanolamine and o-vanillin precursors. The X-ray crystallography of the complexes were reported. Both complexes had same ligands in their structures with different environment around transition metal ions. Different experimental such as absorption studies, cyclic voltammetry, ethidium bromide displacement studies and viscosity measurement besides of docking method were used to study the binding of the complexes to DNA. Based on these studies, intercalation was proposed to be the main mode of binding for both complexes and CuII complex was a better intercalator in interaction with DNA than CoIII complex. Also, CuII complex triggered double strand break in presence of oxidized agent, H2O2. The MTT and Apoptosis/necrosis analysis were revealed that CuII complex induced apoptosis as compared with anticancer agent, Cisplatin.& COPY; 2023 Elsevier B.V. All rights reserved.

Automated summary

Two new Schiff base mononuclear CuII and CoIII complexes were synthesized using monoethanolamine and o-vanillin as precursors. X-ray crystallography was used to study the structures of the complexes. Various experiments were conducted to analyze the binding of the complexes to DNA, and it was found that intercalation is the main mode of binding. The CuII complex showed better intercalation and induced double strand break in the presence of H2O2.