4.4 Article

Quantification of Allantoin in Yams (Dioscorea sp.) Using a 1H NMR Spectroscopic Method

期刊

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 33, 期 5, 页码 662-667

出版社

KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.2301.01009

关键词

Allantoin; yam; Dioscorea; NMR; qNMR; quantitation

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This study developed a 1H quantitative nuclear magnetic resonance (qNMR) method for quantification of allantoin in yams. The method was based on the relative ratio of signals integration of allantoin to the internal standard dimethyl sulfone (DMSO2) and validated in terms of specificity, linearity, sensitivity, precision, and recovery. The method was then applied to evaluate the allantoin content in the flesh and peel extracts of four different yams cultivated in Korea.
Allantoin is an abundant component of yams and has been known as a skin protectant due to its pharmacological activities. In previous methods for allantoin determination using high-performance liquid chromatography (HPLC), the separation was unsatisfactory. We herein developed a 1H quantitative nuclear magnetic resonance (qNMR) method for quantification of allantoin in the flesh and peel of yams. The method was carried out based on the relative ratio of signals integration of allantoin to a certain amount of the internal standard dimethyl sulfone (DMSO2) and validated in terms of specificity, linearity (range 62.5-2000 mu g/ml), sensitivity (limit of detection (LOD) and quantification (LOQ) 4.63 and 14.03 mu g/ml, respectively), precision (RSD% 0.02-0.26), and recovery (86.35-92.11%). The method was then applied for the evaluation of allantoin in flesh and peel extracts of four different yams cultivated in Korea.

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