4.4 Article

Unraveling Biohydrogen Production and Sugar Utilization Systems in the Electricigen Shewanella marisflavi BBL25

期刊

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 33, 期 5, 页码 687-697

出版社

KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.2212.12024

关键词

Shewanella; whole-genome sequencing; biohydrogen production; carbon source utilization system

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This study identified genes associated with carbon source utilization, electron transfer systems, and hydrogen production systems in Shewanella marisflavi BBL25 through whole-genome sequencing. In addition, introducing the glk gene from S. marisflavi BBL25 into S. oneidensis MR-1 improved glucose consumption. The findings contribute to the understanding of sugar utilization, electron transfer systems, and hydrogen production systems in other Shewanella species.
Identification of novel, electricity-producing bacteria has garnered remarkable interest because of the various applications of electricigens in microbial fuel cell and bioelectrochemical systems. Shewanella marisflavi BBL25, an electricity-generating microorganism, uses various carbon sources and shows broader sugar utilization than the better-known S. oneidensis MR-1. To determine the sugar-utilizing genes and electricity production and transfer system in S. marisflavi BBL25, we performed an in-depth analysis using whole-genome sequencing. We identified various genes associated with carbon source utilization and the electron transfer system, similar to those of S. oneidensis MR-1. In addition, we identified genes related to hydrogen production systems in S. marisflavi BBL25, which were different from those in S. oneidensis MR-1. When we cultured S. marisflavi BBL25 under anaerobic conditions, the strain produced 427.58 +/- 5.85 mu l of biohydrogen from pyruvate and 877.43 +/- 28.53 mu l from xylose. As S. oneidensis MR-1 could not utilize glucose well, we introduced the glk gene from S. marisflavi BBL25 into S. oneidensis MR-1, resulting in a 117.35% increase in growth and a 17.64% increase in glucose consumption. The results of S. marisflavi BBL25 genome sequencing aided in the understanding of sugar utilization, electron transfer systems, and hydrogen production systems in other Shewanella species.

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