Characterizing the Myoarchitecture of the Supraspinatus and Infraspinatus Muscles With MRI Using Diffusion Tensor Imaging

Background: The societal cost of shoulder disabilities in our aging society keeps rising. Providing biomarkers of early changes in the microstructure of rotator cuff (RC) muscles might improve surgical planning. Elevation angle (E1A) and pen nation angle (PA) assessed by ultrasound change with RC tears. Furthermore, ultrasounds lack repeatability. Purpose: To propose a repeatable framework to quantify the myocyte angulation in RC muscles. Study Type: Prospective. Subjects: Six asymptomatic healthy volunteers (1 female aged 30 years; 5 males, mean age 35 years, range 25-49 years), who underwent three repositioned scanning sessions (10 minutes apart) of the right infraspinatus muscle (ISPM) and supraspinatus muscle (SSPM). Field Strength/Sequence: 3-T, T1-weighted and diffusion tensor imaging (DTI; 12 gradient encoding directions, b-values of 500 and 800 s/mm(2)). Assessment: Each voxel was binned in percentage of depth defined by the shortest distance in the antero-posterior direction (manual delineation), i.e. the radial axis. A second order polynomial fit for PA across the muscle depth was used, while E1A described a sigmoid across depth: E1A(sig) = E1A(range) x sigmf 1 : 100%depth, -EA1(grad) , E1A(asym) + E1A(shift). Statistical Tests: Repeatability was assessed with the nonparametric Wilcoxon's rank-sum test for paired comparisons across repeated scans in each volunteer for each anatomical muscle region and across repeated measures of the radial axis. A P-value <0.05 was considered statistically significant. Results: In the ISPM, E1A was constantly negative, became helicoidal, then mainly positive across the antero-posterior depth, respective at the caudal, central and cranial regions. In the SSPM, posterior myocytes ran more parallel to the intramuscular tendon (PA approximate to - 0 degrees), while anterior myocytes inserted with a pennation angle (PA approximate to- 20 degrees). E1A and PA were repeatable in each volunteer (error < 10%). Intra-repeatability of the radial axis was achieved (error < 5%). Data Conclusion: ElA and PA in the proposed framework of the ISPM and SSPM are repeatable with DTI. Variations of myocyte angulation in the ISPM and SSPM can be quantified across volunteers.

Automated summary

In this study, a repeatable method was proposed to quantify the myocyte angulation in rotator cuff (RC) muscles. By scanning the right infraspinatus muscle (ISPM) and supraspinatus muscle (SSPM), the variations of myocyte angulation were analyzed. The results showed that E1A changed helically with depth in the ISPM, while the pennation angle (PA) exhibited significant differences between the anterior and posterior insertion points in the SSPM. Furthermore, DTI was proven to be capable of achieving repeatable measurements of E1A and PA.