Biochar-immobilized Bacillus megaterium enhances Cd immobilization in soil and promotes Brassica chinensis growth

Phosphate solubilizing bacteria (PSB) has been considered an environmental-friendly phosphate fertilizer without cadmium (Cd) input into soils, but its possibility of Cd fixation in soil needs to be explored. Since direct inoculation results in a rapid decline of the population and activity, we immobilized Bacillus megaterium with maize straw biochar (B-PSB) and investigated its feasibility in remediating Cd-contaminated soil. Pot experiments showed that the application of B-PSB significantly ameliorated the growth of Brassica chinensis under Cd stress, with a fresh weight increased by 59.08% compared to the Cd-control. B-PSB reduced Cd accumulation in Brassica chinensis by 61.69%, and promoted the uptake of P and N by 134.97% and 98.71% respectively. Microbial community analysis showed B-PSB recruited more plant growth-promoting bacteria in near-rhizosphere soil, which provides a favorable microenvironment for both PSB and crops. Column leaching experiments verified that B-PSB achieved the dissolution of stable P while fixing Cd. Batch tests further revealed that biochar served as a successful carrier facilitating the growth of B. megaterium and Cd immobilization. Given the widespread Cd contamination in agricultural soils, our results indicate that B-PSB is a promising soil amendment to secure food safety.

Automated summary

Phosphate solubilizing bacteria (PSB) is an environmentally friendly phosphate fertilizer without cadmium (Cd) input into soils. However, its potential for Cd fixation in soil needed to be explored. In this study, Bacillus megaterium was immobilized with maize straw biochar (B-PSB) and found to successfully remediate Cd-contaminated soil, promoting the growth of Brassica chinensis and reducing Cd accumulation in the plants.