期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 74, 期 13, 页码 3806-3820出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erad072
关键词
CRISPR-Cas; DNA sequence knock-in; DNA sequence replacement; homologous recombination; plant gene targeting
This paper reviews recent advances in CRISPR/Cas-mediated gene targeting in plants, discusses their potential applications, and suggests further improvements to achieve higher efficiency.
A review of recent advances in CRISPR/Cas-mediated gene targeting in plants, discussing their potential applications, and suggesting further improvements for achieving higher efficiency. Gene targeting can be used to make modifications at a specific region in a plant's genome and create high-precision tools for plant biotechnology and breeding. However, its low efficiency is a major barrier to its use in plants. The discovery of CRISPR (clustered regularly interspaced short palindromic repeats)-Cas-based site-specific nucleases capable of inducing double-strand breaks in desired loci resulted in the development of novel approaches for plant gene targeting. Several studies have recently demonstrated improvements in gene targeting efficiency through cell-type-specific expression of Cas nucleases, the use of self-amplified gene-targeting-vector DNA, or manipulation of RNA silencing and DNA repair pathways. In this review, we summarize recent advances in CRISPR/Cas-mediated gene targeting in plants and discuss potential efficiency improvements. Increasing the efficiency of gene targeting technology will help pave the way for increased crop yields and food safety in environmentally friendly agriculture.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据