期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 304, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2022.116028
关键词
Koumine; IPEC-J2; Inflammation; ERS; ROS; Apoptosis; Autography; NF-?B; PI3K
Koumine, an indole alkaloid, has anti-inflammatory and antioxidant effects and can protect the intestine from injury. It mitigates H2O2-induced cell damage by enhancing cell viability and suppressing cell membrane fragmentation, as well as inhibiting the production of inflammatory factors. Additionally, koumine protects cells by preventing apoptosis and autophagy.
Ethnopharmacological relevance: Koumine, an indole alkaloid extracted from Gelsemium elegans Benth, exerts anti-inflammation and antioxidant activities. However, the effects of koumine on intestinal injury induced by H2O2 and its potential molecular mechanisms need larger studies. Aim of the study: We established an IPEC-J2 cell damage model induced by H2O2 to explore the protective mechanism of koumine on intestinal injury.Materials and methods: In the experiment, cell damage models were made with hydrogen peroxide. To assess the protective effect of koumine on H2O2-induced IPEC-J2 cell injury, CCK-8, the release of LDH and ROS, trans-mission electron microscopy and Annexin V-FITC/PI were employed. Western Blot and Quantitative Real-time PCR were used to determine the potential alleviated mechanism of koumine on H2O2-trigged IPEC-J2 cell damage.Results: The results of CCK-8 and LDH implied that koumine has a mitigative effect on H2O2-induced cell damage via upregulating cell viability and suppressing cell membrane fragmentation. Simultaneously, koumine notably inhibited the level of pro-inflammatory factors (IL-1 beta, IL-6, IL-8, TNF-alpha and TGF-beta), the over-production of ROS along with decreasing the injury of mitochondrion, endoplasmic reticulum and lysosome induced by H2O2. Moreover, koumine dramatically attenuated H2O2-triggered IPEC-J2 cell apoptosis and autophagy. Subse-quently, Western blot analysis identified NF-Kappa B, PI3K and ERS as possible pathway responsible for the protective effect of koumine on H2O2-stimulated IPEC-J2 cell inflammation.
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