期刊
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
卷 261, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jenvrad.2023.107141
关键词
Tritium; Zebrafish Danio rerio; Early -life stages; Transcriptomics; Proteomics
In this study, the effects of tritiated water (HTO) on zebrafish larvae were investigated. Transcriptomic and proteomic analyses revealed similar impacts on biological pathways such as defence response, muscle integrity and contraction, and potential visual alterations. Interestingly, these effects partly overlapped with those induced by gamma irradiation, suggesting potential common modes of action. This study provides evidence of the molecular-level effects of HTO on zebrafish larvae, and further research could explore whether these effects persist in adult organisms.
In the environment, populations are exposed to different kinds of ionizing radiation. Little is known about their modes of action on non-human species, and whether or not they are similar for alpha, beta and gamma radia-tions, considered as the reference. In this context, tritium effects (beta emitter) under the form of tritiated water (HTO) were investigated in zebrafish, a common model in toxicology and ecotoxicology with a fully sequenced genome. Experiments were conducted on early life stages, considered to be highly sensitive to pollutants, by exposing eggs to 0.4 mGy/h of HTO until 10 days post fertilization. Tritium internalization was quantified, and effects were investigated using a combined approach of transcriptomic and proteomic analyses. Results high-lighted similarities in the biological pathways affected by HTO by both techniques, such as defence response, muscle integrity and contraction, and potential visual alterations. These results correlated well with previous data obtained on earlier developmental stages (1 and 4 dpf). Interestingly, HTO effects were partly overlapping those obtained after gamma irradiation, underlying potential common modes of action. This study, therefore, brought a body of evidence on the effects of HTO observed at the molecular level on zebrafish larvae. Further studies could investigate if the effects persist in adult organisms.
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