Steroid hormone-dependent changes in trehalose physiology in the silkworm, Bombyx mori

Holometabolous insects undergo metamorphosis to reconstruct their body to the adult form during pupal period. Since pupae cannot take any diets from the outside because of a hard pupal cuticle, those insects stock up on nutrients sufficient for successful metamorphosis during larval feeding period. Among those nutrients, carbohydrates are stored as glycogen or trehalose, which is the major blood sugar in insects. The hemolymph trehalose is constantly high during the feeding period but suddenly decreases at the beginning of the prepupal period. It is believed that trehalase, which is a trehalose-hydrolyzing enzyme, becomes highly active to reduce hemolymph trehalose level during prepupal period. This change in the hemolymph trehalose level has been interpreted as the physiological shift from storage to utilization of trehalose at that stage. Although this shift in trehalose physiology is indispensable for energy production required for successful metamorphosis, little is known on the regulatory mechanisms of trehalose metabolism in accordance with developmental progress. Here, we show that ecdysone, an insect steroid hormone, plays essential roles in the regulation of soluble trehalase activity and its distribution in the midgut of silkworm, Bombyx mori. In the end of larval period, soluble trehalase was highly activated in the midgut lumen. This activation was disappeared in the absence of ecdysone and also restored by ecdysone administration. Our present results suggest that ecdysone is essentially required for the changes in the function of the midgut on trehalose physiology as development progresses.

Automated summary

Holometabolous insects undergo metamorphosis during pupal period to reconstruct their body to the adult form. Carbohydrates, stored as glycogen or trehalose, play a major role in providing nutrients for successful metamorphosis. The hormone ecdysone is essential for regulating trehalose metabolism in the midgut, where trehalase activity is highly activated. The absence of ecdysone results in the loss of trehalase activation, while ecdysone administration restores it. This study highlights the importance of ecdysone in the regulation of midgut function and trehalose physiology during development.