期刊
JOURNAL OF CLINICAL VIROLOGY
卷 160, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jcv.2022.105377
关键词
Rubella; Rubella virus; Virus shedding; Viral load; RT-qPCR; Real-time RT-PCR; Virus isolation; Infection control
类别
This study analyzed samples from patients with rubella and found that virus shedding peaked 0-2 days after rash onset and decreased over time. The viral RNA load and presence of infectious rubella virus were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and virus isolation. These findings contribute to improved algorithms for rubella surveillance and diagnosis.
Background: Since the first isolation of rubella virus (RuV) in 1962, comprehensive data regarding the quanti-tative evaluation of RuV shedding remain unavailable. In this study, we evaluated the shedding of viral RNA and infectious virus in patients with acute RuV infection.Study design: We analyzed 767 specimens, including serum/plasma, peripheral blood mononuclear cells (PBMCs), throat swabs, and urine, obtained from 251 patients with rubella. The viral RNA load and presence of infectious RuV were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and virus isolation.Results: Virus excretion peaked 0-2 days after rash onset and decreased over time. The median viral RNA load dropped to an undetectable level on day 3 after rash onset in serum/plasma, day 2 in PBMCs, days 10-13 in throat swabs, and days 6-7 in urine. Infectious virus could be isolated for up to day 2 after rash onset in serum/ plasma, day 1 in PBMCs, days 8-9 in throat swabs, and days 4-5 in urine. The minimum viral RNA load that allowed virus isolation was 961 copies/mL in serum/plasma, 784 copies/mL in PBMCs, 650 copies/mL in throat swabs, and 304 copies/mL in urine. A higher viral RNA load indicated a higher likelihood of the presence of infectious virus.Conclusion: These findings would contribute to improve algorithms for rubella surveillance and diagnosis. In addition, this study indicates that the results of RT-qPCR enable efficient rubella control by estimating candidate patients excreting infectious virus, which could help prevent viral transmission at an early stage and eliminate rubella ultimately.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据