期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 -, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0271678X231173584
关键词
Brain metabolism; 1; 2-C-13(2) glucose; microdialysis; NMR; traumatic brain injury (human)
The optimization of glucose metabolism in the injured human brain remains uncertain, particularly with regard to the ability of the injured brain to metabolize additional glucose. This study investigated the impact of microdialysis-delivered 1,2-C-13(2) glucose at different concentrations on brain extracellular chemistry in traumatised patients. The results indicated that there may be metabolic factors influencing the extracellular chemistry changes, and a large proportion of extracellular lactate does not originate from local glucose metabolism.
How to optimise glucose metabolism in the traumatised human brain remains unclear, including whether injured brain can metabolise additional glucose when supplied. We studied the effect of microdialysis-delivered 1,2-C-13(2) glucose at 4 and 8 mmol/L on brain extracellular chemistry using bedside ISCUSflex, and the fate of the C-13 label in the 8 mmol/L group using high-resolution NMR of recovered microdialysates, in 20 patients. Compared with unsupplemented perfusion, 4 mmol/L glucose increased extracellular concentrations of pyruvate (17%, p = 0.04) and lactate (19%, p = 0.01), with a small increase in lactate/pyruvate ratio (5%, p = 0.007). Perfusion with 8 mmol/L glucose did not significantly influence extracellular chemistry measured with ISCUSflex, compared to unsupplemented perfusion. These extracellular chemistry changes appeared influenced by the underlying metabolic states of patients' traumatised brains, and the presence of relative neuroglycopaenia. Despite abundant C-13 glucose supplementation, NMR revealed only 16.7% C-13 enrichment of recovered extracellular lactate; the majority being glycolytic in origin. Furthermore, no C-13 enrichment of TCA cycle-derived extracellular glutamine was detected. These findings indicate that a large proportion of extracellular lactate does not originate from local glucose metabolism, and taken together with our earlier studies, suggest that extracellular lactate is an important transitional step in the brain's production of glutamine.
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