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A phosphatidylinositol phosphate kinase inhibits Ras activation and regulates chemorepulsion in Dictyostelium discoideum

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JOURNAL OF CELL SCIENCE
卷 136, 期 14, 页码 -

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COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.260541

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KEY WORDS; AprA; Dictyostelium discoideum; Phagocytosis; Phosphatidylinositol; Ras; Chemorepulsion

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During developmental and immune responses, cells can move towards or away from signals. The movement of cells away from a stimulus, known as chemorepulsion, is poorly understood. In this study, researchers found that a protein called AprA is secreted by cells to repel other cells. They also identified an enzyme called PIPkinA that is necessary for chemorepulsion. PIPkinA controls the activation of a protein called Ras and regulates the movement of cells away from AprA.
During developmental and immune responses, cells move towards or away from some signals. Although much is known about chemoattraction, chemorepulsion (the movement of cells away from a stimulus) remains poorly understood. Proliferating Dictyostelium discoideum cells secrete a chemorepellent protein called AprA. Examining existing knockout strains, we previously identified proteins required for AprA-induced chemorepulsion, and a genetic screen suggested that the enzyme phosphatidylinositol phosphate kinase A (PIPkinA, also known as Pik6) might also be needed for chemorepulsion. Here, we show that cells lacking PIPkinA are not repelled by AprA, and that this phenotype is rescued by expression of PIPkinA. To bias cell movement, AprA inhibits Ras activation at the side of the cell closest to the source of AprA, and we find that PIPkinA is required for AprA to inhibit Ras activation. PIPkinA decreases levels of phosphatidylinositol 4-phosphate [PI(4)P] and phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3], and possibly because of these effects, potentiates phagocytosis and inhibits cell proliferation. Cells lacking PIPkinA show normal AprA binding, suggesting that PIPkinA regulates chemorepulsion at a step between the AprA receptor and AprA inhibition of Ras activation.

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