Skeletal muscle aging and sarcopenia: Perspectives from mechanical studies of single permeabilized muscle fibers

The decline in muscle mass and strength with age is well documented and associated with weakness, decreased flexibility, vulnerability to diseases and/or injuries, and impaired functional restoration. The term sarcopenia has been used to refer to the loss of muscle mass, strength and impaired physical performance with advanced adult age and recently has become a major clinical entity in a super-aged society. To understand the pathophysiology and clinical manifestations of sarcopenia, it is essential to explore the age-related changes in the intrinsic properties of muscle fibers. Mechanical experiments with single muscle fibers have been conducted during the last 80 years and applied to human muscle research in the last 45 years as an in-vitro muscle function test. Fundamental active and passive mechanical properties of skeletal muscle can be evaluated using the isolated permeabilized (chemically skinned) single muscle fiber preparation. Changes in the intrinsic properties of older human single muscle fibers can be useful biomarkers of aging and sarcopenia. In this review, we summarize the historical development of single muscle fiber mechanical studies, the definition and diagnosis of muscle aging and sarcopenia, and age-related change of active and passive mechanical properties in single muscle fibers and discuss how these changes can be used to assess muscle aging and sarcopenia.

Automated summary

The decline in muscle mass and strength with age, known as sarcopenia, is a major clinical concern in an aging society. Understanding the changes in muscle fibers is essential in studying sarcopenia. Mechanical experiments with single muscle fibers have been conducted for many years and can serve as useful biomarkers for aging and sarcopenia. This review summarizes the historical development of these studies, the definition and diagnosis of muscle aging and sarcopenia, and the age-related changes in mechanical properties of single muscle fibers.