Effect of size and morphology of functionalized upconversion nanoparticle labeled probe on stem cell absorption

In order to improve the application of stem cell therapy, it is important to monitor the proliferation and differentiation of transplanted stem cells in real time, high sensitivity and high precision. Upconversion nanoparticles (UCNPs) have been employed as bioimaging agents and delivery vehicles for gene therapeutics in several types of cells. Herein, we fabricated multiple functional upconversion nanoparticles (FUC-NPs) as exogenous contrast agents to investigate the effect of FUC-NPs size on the efficiency of tracking human umbilical cord mesenchymal stem cells (HUCMSCs). We used TEM, DLS, MTT, etc. systematically detected the effects of nanoparticle dose and exposure time on HUCMSCs cytotoxicity. Our data showed that incubation of FUC-NPs, HUCMSCs are able to maintain their viability and differentiation ability. Interestingly, the small size FUC-NPs examined, S-FUCs, had a much higher uptake capability than the other FUC-NPs (B-FUCs, H-FUCs and R-FUCs).The study will aid in UCNPs for the engineering of stem cell application in the future.

Automated summary

To improve the application of stem cell therapy, the authors used upconversion nanoparticles (UCNPs) as contrast agents to track human umbilical cord mesenchymal stem cells (HUCMSCs). The data showed that incubation of UCNPs allowed HUCMSCs to maintain their viability and differentiation ability, with smaller sized UCNPs demonstrating higher uptake capability. This study will contribute to the future engineering of stem cell applications using UCNPs.