期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 78, 期 5, 页码 1288-1294出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkad091
关键词
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A new carbapenem-resistant Escherichia coli strain was found and a novel carbapenemase, OXA-1041, was identified. OXA-1041 showed preferential activity against ertapenem and was encoded on a self-transmissible plasmid. The study provides important insights into the genetic basis of carbapenem resistance.
Background We found a carbapenem-resistant Escherichia coli without known carbapenemase-encoding genes and performed a study to identify the possible new carbapenemase. Methods The production of carbapenemase was examined using the modified carbapenem inactivation method. The strain was subjected to short- and long-read genome sequencing and the complete genome was obtained by hybrid assembly. The gene encoding a potential new OXA-type carbapenemase was cloned. The enzyme was purified and was then subjected to kinetic assays. Molecular docking analysis of the enzyme was performed using the MOE software suite. Mating experiments were attempted to obtain the plasmid carrying the corresponding gene. Results We identified and characterized a novel class D carbapenem-hydrolysing beta-lactamase, OXA-1041, in a carbapenem-resistant E. coli clinical strain. OXA-1041 had 89.77% (237/264) amino acid identity with OXA-427, a known carbapenemase. By cloning in an E. coli laboratory strain, bla(OXA-1041) was found to reduce susceptibility to ertapenem by 16 times (MIC 0.25 versus 0.016 mg/L) and meropenem by four times (MIC 0.06 versus 0.016 mg/L) but did not significantly reduce susceptibility to imipenem and doripenem. Enzyme kinetic measurement of purified OXA-1041 showed that OXA-1041 could hydrolyse ertapenem and meropenem with a turnover number (k(cat))/Michaelis constant (K-M) of 8.57 and 3.63 mM(-1)s(-1), respectively. The complete genome contained a single plasmid (223 341 bp, IncF, containing five replicons), which was self-transmissible. bla(OXA-1041) was downstream of insertion sequence ISCR1 and there were three tandem copies of ISCR1-bla(OXA-1041)-creD Delta (encoding an envelope protein) on this plasmid. Conclusions The above findings suggest OXA-1041 is a new plasmid-encoded carbapenemase with preferential activity against ertapenem.
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