期刊
JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 13, 期 124, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsif.2016.0688
关键词
three-dimensional matrigel; altered gravity; capillary-like structures; cytoskeleton; hypergravity pre-stimulation
资金
- Spin Your Thesis! programme
- European Regional Development Fund (ERDF) through the Programa Operacional Factores de Competitividade - COMPETE
- FCT-Fundacao para a Ciencia e a Tecnologia in the framework of FCT-POPH-FSE
- North Portugal Regional Operational Programme (ON.2-O Novo Norte) under the National Strategic Reference Framework (NSRF) [NORTE-01-0145-FEDER-000012]
- [PEst-C/SAU/LA0002/2011]
- Fundação para a Ciência e a Tecnologia [PEst-C/SAU/LA0002/2011] Funding Source: FCT
Angiogenesis, the formation of blood vessels from pre-existing ones, is a key event in pathology, including cancer progression, but also in homeostasis and regeneration. As the phenotype of endothelial cells (ECs) is continuously regulated by local biomechanical forces, studying endothelial behaviour in altered gravity might contribute to new insights towards angiogenesis modulation. This study aimed at characterizing EC behaviour after hypergravity exposure (more than 1g), with special focus on cytoskeleton architecture and capillary-like structure formation. Herein, human umbilical vein ECs (HUVECs) were cultured under two-dimensional and three-dimensional conditions at 3g and 10g for 4 and 16 h inside the large diameter centrifuge at the European Space Research and Technology Centre (ESTEC) of the European Space Agency. Although no significant tendency regarding cytoskeleton organization was observed for cells exposed to high g's, a slight loss of the perinuclear localization of beta-tubulin was observed for cells exposed to 3g with less pronounced peripheral bodies of actin when compared with 1g control cells. Additionally, hypergravity exposure decreased the assembly of HUVECs into capillary-like structures, with a 10g level significantly reducing their organization capacity. In conclusion, short-term hypergravity seems to affect EC phenotype and their angiogenic potential in a time and g-level-dependent manner.
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