An Antenna-Abundant Glutathione S-Transferase BdGSTd8 Participates in Detoxification of Two Organophosphorus Insecticides in Bactrocera dorsalis (Hendel)

The oriental fruit fly, Bactrocera dorsalis, isa damaging insect pest for many vegetable and fruit crops that hasevolved severe chemical insecticide resistance, including organophosphorus,neonicotinoid, pyrethroid, and macrolides. Hence, it is importantto elucidate its detoxification mechanism to improve its managementand mitigate resource destruction. Glutathione S-transferase(GST) is a critical secondary phase enzyme that plays multiple detoxificationfunctions against xenobiotics. In this study, we identified severalBdGSTs by characterizing their potential relationships with five insecticidesusing inducible and tissue-specific expression pattern analyses. Wefound that an antenna-abundant BdGSTd8 respondedto four different classes of insecticides. Subsequently, our immunohistochemicaland immunogold staining analysis further confirmed that BdGSTd8 wasprimarily located in the antenna. Our investigations also confirmedthat BdGSTd8 possesses the capability to enhance cell viability bydirectly interacting with malathion and chlorpyrifos, which clarifiedthe function of antenna-abundant GST in B. dorsalis. Altogether, these findings enrich our understanding of GST molecularcharacteristics in B. dorsalis and provide new insightsinto the detoxification of superfluous xenobiotics in the insect antenna.

Automated summary

The oriental fruit fly, Bactrocera dorsalis, is a damaging insect pest that has developed resistance to various chemical insecticides. This study identified several glutathione S-transferases (GSTs) in B. dorsalis and characterized their relationships with five insecticides. It was found that an antenna-abundant BdGSTd8 responded to four different classes of insecticides. Further analysis confirmed that BdGSTd8 is primarily located in the antenna and has the ability to enhance cell viability by interacting with specific insecticides. These findings enhance our understanding of GST characteristics in B. dorsalis and their role in detoxification.