4.7 Article

Colonization Mechanism of Endophytic Enterobacter cloacae TMX-6 on Rice Seedlings Mediated by Organic Acids Exudated from Roots

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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 71, 期 12, 页码 4802-4809

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AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c08647

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root exudates; rhizosphere directional regulation; thiamethoxam; chemotaxis; biofilm formation

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Through targeted metabolomics analysis, 20 main small molecular organic acids (SMOAs) in rice root exudates were found to differ significantly between the control group and the 10 mg/L thiamethoxam-treated group. Among these, malic acid, citric acid, succinic acid, and proline were found to induce chemotactic response, swimming ability, and biofilm formation of Enterobacter cloacae TMX-6 in a dose-dependent manner. It was confirmed that the addition of these four compounds promoted the colonization of TMX-6. This study provides important insights for directional regulation of beneficial plant-microbe interactions.
Small molecular organic acids (SMOAs) in root exudates are critical for plant-microbe interaction, especially under environmental stresses. However, the dominant organic acids driving the process and promoting the colonization are unclear. Here, using a target metabolomics, 20 main SMOAs of rice root exudates were identified and analyzed in control and 10 mg/L thiamethoxam-treated groups. The composition of these SMOAs differed significantly between the two treatments. Among which, malic acid, citric acid, succinic acid, and proline induced a chemotactic response, swimming ability, and biofilm formation of Enterobacter cloacae TMX-6 in a dose-dependent manner. The maximal chemotactic response of TMX-6 was induced by proline at 10 mg/L, and a strong chemotactic response was even observed at 0.01 mg/L. The recruitment assay confirmed that the addition of these four compounds promoted the colonization of TMX-6. The results provide insight for directional regulation of plant-microbe interactions for beneficial outcomes.

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