Tahibacter harae sp. nov., isolated from pig farm soil in Guangdong, PR China

In the process of exploring the microbial diversity of pig farms, a Gram- stain-negative, aerobic, rod-shaped, non- spore-forming, non-motile bacterial strain, designated P2K(T), was isolated from soil sample collected at a pig farm, Guangdong Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P2K(T) belonged to the genus Tahibacter, with the highest sequence similarity to Tahibacter aquaticus PYM5-11(T) (98.6%) and Tahibacter caeni BUT-6(T) (98.3 %). The genome size of strain P2K(T) was 6.0 Mb with a DNA G+C content of 68.3 mol%. Average nucleotide identity values between strain P2K(T) and the type strains of the genus Tahibacter were 81.1-81.6 %. The digital DNA-DNA hybridization values between P2K(T) and these relative species were 24.5-25.6%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, an unknown aminolipids, two unknown lipids and three unknown phospholipids. The major respiratory quinone of strain P2K(T) was ubiqui-none Q-8, and the main fatty acids (>10.0 % of total fatty acids) of strain P2K(T) were iso-C-15:0, iso-C-16:0 and summed feature 9 (C-16:0 10-methyl and/or iso-C-17:1 omega 9c). Based on phenotypic and genotypic data, strain P2K(T) represents a novel species within the genus Tahibacter, for which the name Tahibacter harae sp. nov. is proposed, with the type strain P2K(T) (=GDMCC 1.3107(T)=JCM 35231(T)).

Automated summary

A novel bacterial strain, designated P2K(T), was isolated from a soil sample collected at a pig farm in Guangdong Province, PR China. The strain was identified as Tahibacter harae based on phenotypic and genotypic analysis. The genome size of strain P2K(T) was 6.0 Mb with a DNA G+C content of 68.3 mol%. This study expands our knowledge of the microbial diversity in pig farms, specifically in relation to the genus Tahibacter.