Gaoshiqia sediminis gen. nov., sp. nov., isolated from coastal sediment

A Gram- stain-negative, facultative anaerobic, motile, rod-shaped and orange bacterium, designated A06T, was obtained off the coast of Weihai, PR China. Cells were 0.4-0.5x0.6-1.0 mu m in size. Strain A06T grew at 20-40 degrees C (optimum, 33 degrees C), at pH 6.0-8.0 (optimum, pH 6.5-7.0) and in the presence of 0-8 % NaCl (w/v) (optimum, 2 %). Cells were oxidase and catalase positive. Menaquinone- 7 was detected as the major respiratory quinone. The dominant cellular fatty acids were identi-fied as C15:0 2-OH, iso-C15:0, anteiso-C15:0 and iso-C15:1 omega 6c. The DNA G+C content of strain A06T was 46.1 mol%. The polar lipids were phosphatidylethanolamine, one aminolipid, one glycolipid and three unidentified lipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A06T is a member of the family Prolixibacteraceae and exhibited the highest sequence similarity to Mangrovibacterium diazotrophicum DSM 27148T (94.3 %). Based on its phylogenetic and phenotypic characteristics, strain A06T is considered to represent a novel genus in the family Prolixibacteraceae, for which the name Gaoshiqia gen. nov. is proposed. The type species is Gaoshiqia sediminis sp. nov., with type strain A06T (=KCTC 92029T=MCCC 1H00491T). The identification and acquisition of microbial species and genes in sediments will help broaden the understanding of microbial resources and lay a foundation for its application in biotechnology. Strain A06T uses an enrichment method, so the isolation of strain A06T is of great significance to the enrichment of marine microbial resources.

Automated summary

A Gram-stain-negative, orange, rod-shaped bacterium named A06T was isolated off the coast of Weihai, PR China. Strain A06T grew at temperatures between 20-40 degrees C, pH 6.0-8.0, and 0-8% NaCl concentration. Phylogenetic analysis showed that A06T belongs to the family Prolixibacteraceae and is a novel genus named Gaoshiqia gen. nov. The isolation of strain A06T is significant for the enrichment of marine microbial resources.