Paenibacillus sedimenti sp. nov., isolated from freshwater wetland sediment

A Gram- stain-positive, motile, rod-shaped, facultatively anaerobic bacterium, designated strain WST5T, isolated from sediment was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WST5T was most closely related to Paenibacillus aestuarii CJ25T (96.8 % similarity). The genome size of the WST5T was 6.5 Mb, contained 4500 predicted protein-coding genes, and had a DNA G+C content of 46.6%. The values of whole-genome average nucleotide iden-tity analysis and digital DNA-DNA hybridization between strain WST5T and its closely related type strains were less than 76 and 25.6 %, respectively. The predominant cellular fatty acids (>10 %) were anteiso- C15: 0 and C16:1 & omega;5c and the main menaquinone was MK -7. The major polar lipids were identified as diphospholidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminophospholipids. Based on the results of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain WST5T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus sedimentum sp. nov. is proposed. The type strain is WST5T (=NBRC 115194 T=CGMCC 1.18706T).

Automated summary

A newly isolated bacterium, strain WST5T, was identified as a Gram-stain-positive, motile, rod-shaped, and facultatively anaerobic bacterium. Phylogenetic analysis revealed its close relationship with Paenibacillus aestuarii CJ25T, with a similarity of 96.8%. The strain's genome size was 6.5 Mb, containing predicted protein-coding genes and having a G+C content of 46.6%. Based on multiple analyses, strain WST5T is proposed as a novel species of the genus Paenibacillus, named Paenibacillus sedimentum sp. nov.