4.7 Article

Diffusion coefficient of cationic liposomes during lipoplex formation determines transfection efficiency in HepG2 cells

期刊

出版社

ELSEVIER
DOI: 10.1016/j.ijpharm.2023.122881

关键词

Diffusion coefficient; Cationic liposome; Lipoplex formation; Transfection efficiency; Endocytosis pathway

向作者/读者索取更多资源

Cationic lipid-based lipoplexes were prepared with different sizes and incubated with plasmid DNA at different temperatures to investigate the relationship between physicochemical characteristics and transfection efficiency. The diffusion coefficient of liposomes during lipoplex formation was found to strongly correlate with the physicochemical characteristics of lipoplexes, accessibility of plasmid DNA in lipoplexes, and gene expression per metabolic activity. Clathrin-mediated endocytosis was the major route for lipoplexes comprising 100 nm-liposomes, but as liposome size increased, the major route shifted to lipid raft-mediated endocytosis, with macropinocytosis observed for all liposome sizes. The role of reactive oxygen species may depend on liposome size and endocytosis. This study provides valuable information for understanding cationic lipoplex-mediated transfection.
Cationic lipid-based lipoplexes are well-known for gene delivery. To determine the relationship between phys-icochemical characteristics and transfection efficiency, cationic liposomes of different sizes were prepared and incubated with plasmid DNA at different temperatures to form lipoplexes. We found that the liposome diffusion coefficient during lipoplex formation strongly correlated with the physicochemical characteristics of lipoplexes, accessibility of plasmid DNA in lipoplexes, and logarithm of gene expression per metabolic activity. Clathrin-mediated endocytosis was the major route for lipoplexes comprising 100 nm-liposomes, as reported previ-ously. As liposome size increased, the major route shifted to lipid raft-mediated endocytosis. In addition, mac-ropinocytosis was observed for all liposome sizes. The role of reactive oxygen species might depend on liposome size and endocytosis. Information from this study would be useful for understanding cationic lipoplex-mediated transfection.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据