COI diversity supports subspecific division in Western European Lestes virens (Charpentier, 1825) (Zygoptera: Lestidae), but hints at further Mediterranean complexity

We analyse COI sequences of 48 specimens of European Lestes virens (Charpen-tier) to explore patterns in genetic diversity including subspecific boundaries and poten-tial glacial refugia. Our haplotype network and phylogenetic analyses reveal three distinct groups in Western and Northern Europe. One group corresponding to the nominate sub-species L. virens virens is confined to the Iberian Peninsula and southwestern France, and one group corresponding to the subspecies L. virens vestalis is found in the rest of west-ern Europe including southern Scandinavia, mainland Italy and the Mediterranean island Sardinia. Surprisingly three specimens from the Mediterranean island Sicily form a highly distinct group in all our analyses. An analysis of molecular variance (AMOVA) confirms that almost all observed genetic variance is explained by variation between these three groups rather than by variation between sample areas or between individuals. We conclude that the subspecific division into L. virens virens and L. virens vestalis is justified, but further studies are needed to determine the status of the populations in Sicily, southeastern Eu-rope, and North Africa. The genetic pattern we find may reflect different glacial refugia: an Iberian/North African refugium for L. virens virens; a potential Italian refugium for L. virens vestalis; and a Sicilian/North African refugium for the Sicilian populations.

Automated summary

We analysed COI sequences of 48 European Lestes virens specimens to study genetic diversity patterns, including subspecific boundaries and glacial refugia. Our findings revealed three distinct groups in Western and Northern Europe, with one group confined to the Iberian Peninsula and southwestern France, one group found in the rest of western Europe, and a highly distinct group on the Mediterranean island Sicily. The majority of genetic variance was explained by variation between these three groups, supporting the subspecific division. Further research is needed to determine the status of populations in Sicily, southeastern Europe, and North Africa, and to explore the potential glacial refugia.