Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis

Article Biochemical Research Methods

DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles

Lennard van Buren et al.

Summary: DisGUVery is an open-source software that automates the detection and analysis of GUVs in microscopy images, enabling high-throughput analysis and promoting quantitative data analysis in synthetic cell research.

ACS SYNTHETIC BIOLOGY (2023)

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Article Chemistry, Analytical

Mimicking Cellular Metabolism in Artificial Cells: Universal Molecule Transport across the Membrane through Vesicle Fusion

Jingjing Zhao et al.

Summary: Mass transport across cell membranes is essential for cellular metabolism. Electrostatically mediated membrane fusion is used to transport small molecules and macromolecules between oppositely charged vesicles, triggering important biological processes such as enzyme reactions and gene expression. These findings provide a crucial step for investigating complex cellular metabolism.

ANALYTICAL CHEMISTRY (2022)

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Article Biochemistry & Molecular Biology

Applying flow cytometry to identify the modes of action of membrane-active peptides in a label-free and high-throughput fashion

Nanna Wichmann et al.

Summary: In this study, a flow cytometric assay was developed to evaluate the interactions between peptides and lipid membranes. The assay is high throughput, label-free, and provides detailed information about different modes of action of the peptides. The results obtained from the assay were validated using traditional methods, demonstrating its reliability and potential for lipid membrane studies.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2022)

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Article Biochemical Research Methods

Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network

Il-Hyung Lee et al.

Summary: A convolutional neural network-based program was developed to efficiently identify and analyze confocal fluorescence image data of giant unilamellar vesicles. This method can automatically perform protein binding analysis and domain phase separation state decision analysis, improving analysis efficiency and accuracy.

BMC BIOINFORMATICS (2022)

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Article Biochemical Research Methods

Programmable Fusion and Differentiation of Synthetic Minimal Cells

Nathaniel J. Gaut et al.

Summary: Synthetic cells can mimic the complexities of live cells with less noise. This study demonstrates key functionalities of synthetic cells and provides tools for engineering and research.

ACS SYNTHETIC BIOLOGY (2022)

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Review Chemistry, Multidisciplinary

Vesicle-based artificial cells: materials, construction methods and applications

Yao Lu et al.

Summary: The construction of artificial cells with specific functions allows exploration of complex biological processes and provides insights into the origins of life. Using bottom-up methods and various materials, the design of artificial cells can be achieved. Additionally, the use of biomimetic materials and different formation technologies can lead to the successful construction of microreactors and artificial cell networks.

MATERIALS HORIZONS (2022)

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Article Biochemistry & Molecular Biology

Photoinducible Azobenzene trimethylammonium bromide (AzoTAB)-mediated giant vesicle fusion compatible with synthetic protein translation reactions

Boying Xu et al.

Summary: Lipid giant vesicles are a versatile model system for studying the physicochemical basis of lipid membrane fusion. This study investigates the fusion of vesicles mediated by a photosensitive surfactant, AzoTAB, which can induce a conformational change through light. The results show that AzoTAB-induced fusion can be quantitatively analyzed and optimized, making it possible to create controllable synthetic cells with fundamental biological functions.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022)

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Article Biochemical Research Methods

Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids

Sarah Wehrum et al.

Summary: This study reports a novel fusogenic lectin complex that arises from changes in protein architecture, providing new insights into lipid-driven fusion mechanisms. The complex can induce hemifusion and fusion, demonstrated by mixing of fluorescently labeled lipids between vesicles and content mixing of liposomes filled with fluorescently labeled dextran. Furthermore, the complex can form hemifusion diaphragms at vesicle-vesicle interfaces.

ACS SYNTHETIC BIOLOGY (2022)

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Article Chemistry, Multidisciplinary

Bending Rigidity, Capacitance, and Shear Viscosity of Giant Vesicle Membranes Prepared by Spontaneous Swelling, Electroformation, Gel-Assisted, and Phase Transfer Methods: A Comparative Study

Hammad A. Faizi et al.

Summary: This study compares the membrane capacitance, bending rigidity, and shear surface viscosity of giant unilamellar vesicles (GUVs) formed by various commonly used methods. The results show that the bending rigidity values are relatively similar across all methods except for agarose hydration. The capacitance values are similar except for vesicles prepared via PVA gel hydration. Membranes prepared by gel-assisted and phase-transfer methods have higher shear viscosity, likely due to remnants of polymers and oils in the lipid bilayer structure.

LANGMUIR (2022)

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Article Chemistry, Multidisciplinary

DNA-assisted selective electrofusion (DASE) of Escherichia coli and giant lipid vesicles

Sho Takamori et al.

Summary: DNA-assisted selective electrofusion (DASE) utilizes membrane-anchored DNA constructs to bring together objects for fusion, and an electric impulse is applied to trigger fusion, combining the efficiency of standard electrofusion and the selectivity of fusogenic nanostructures.

NANOSCALE (2022)

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Article Chemistry, Multidisciplinary

Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles**

Yannik Dreher et al.

Summary: The controlled division of phase-separated GUVs was studied, revealing a required osmolarity ratio of 2 for division and lower osmolarity ratios for asymmetric division. The study also demonstrated that suitable osmolarity changes can be triggered through various methods like water evaporation, enzymatic decomposition of sucrose, or light-induced uncaging.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Multidisciplinary

Biomimetic Curvature and Tension-Driven Membrane Fusion Induced by Silica Nanoparticles

Marcos Arribas Perez et al.

Summary: The study demonstrates that SiO2 NPs can efficiently induce membrane fusion in biomimetic model membranes, likely by increasing lateral membrane tension. Real-time microscopy reveals three distinct mechanistic pathways for membrane fusion. SiO2 NPs show significant potential for inclusion in the synthetic biology toolkit for membrane remodeling and fusion in artificial cells.

LANGMUIR (2021)

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Article Biology

Giant Vesicles Produced with Phosphatidylcholines (PCs) and Phosphatidylethanolamines (PEs) by Water-in-Oil Inverted Emulsions

Boying Xu et al.

Summary: Giant vesicles (GVs) prepared using the water-in-oil inverted emulsion method showed stability during long-term preservation. The highest productivities of GVs were obtained from pools containing POPC, DOPC, DLPE, DOPC:DLPE (7:3), and POPC:DLPE (6:4).

LIFE-BASEL (2021)

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Article Multidisciplinary Sciences

Controlled division of cell-sized vesicles by low densities of membrane-bound proteins

Jan Steinkuehler et al.

NATURE COMMUNICATIONS (2020)

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Article Multidisciplinary Sciences

Controlled Peptide-Mediated Vesicle Fusion Assessed by Simultaneous Dual-Colour Time-Lapsed Fluorescence Microscopy

Nestor Lopez Mora et al.

SCIENTIFIC REPORTS (2020)

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Article Chemistry, Multidisciplinary

Influence of External NaCl Salt on Membrane Rigidity of Neutral DOPC Vesicles

Judith U. De Mel et al.

LANGMUIR (2020)

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Article Materials Science, Biomaterials

Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins

Yannick R. F. Schmid et al.

ADVANCED BIOSYSTEMS (2020)

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Article Chemistry, Multidisciplinary

Membrane Tension-Mediated Growth of Liposomes

Siddharth Deshpande et al.

SMALL (2019)

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Article Biophysics

Asymmetric Bilayers by Hemifusion: Method and Leaflet Behaviors

Thais A. Enokit et al.

BIOPHYSICAL JOURNAL (2019)

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Article Biochemistry & Molecular Biology

Optimization of the Inverted Emulsion Method for High-Yield Production of Biomimetic Giant Unilamellar Vesicles

Akanksha Moga et al.

CHEMBIOCHEM (2019)

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Article Chemistry, Multidisciplinary

Barcoding Biological Reactions with DNA-Functionalized Vesicles

Justin A. Peruzzi et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

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Article Chemistry, Multidisciplinary

Using Imaging Flow Cytometry to Quantify and Optimize Giant Vesicle Production by Water-in-oil Emulsion Transfer Methods

Yuka Matsushita-Ishiodori et al.

LANGMUIR (2019)

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Review Materials Science, Biomaterials

Self-Assembly of Giant Unilamellar Vesicles by Film Hydration Methodologies

Emeline Rideau et al.

ADVANCED BIOSYSTEMS (2019)

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Article Chemistry, Multidisciplinary

Flow Cytometric Analysis To Evaluate Morphological Changes in Giant Liposomes As Observed in Electrofusion Experiments

Takeshi Sunami et al.

LANGMUIR (2018)

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Article Multidisciplinary Sciences

Sculpting and fusing biomimetic vesicle networks using optical tweezers

Guido Bolognesi et al.

NATURE COMMUNICATIONS (2018)

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Article Chemistry, Multidisciplinary

Asymmetric Ionic Conditions Generate Large Membrane Curvatures

Marzieh Karimi et al.

NANO LETTERS (2018)

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Article Chemistry, Physical

Cations induce shape remodeling of negatively charged phospholipid membranes

Z. T. Graber et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2017)

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Article Chemistry, Physical

Membrane properties of giant polymer and lipid vesicles obtained by electroformation and pva gel-assisted hydration methods

T. P. T. Dao et al.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2017)

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Article Chemistry, Multidisciplinary

A DNA-Programmed Liposome Fusion Cascade

Philipp M. G. Loffler et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

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Article Biochemical Research Methods

MorphoLibJ: integrated library and plugins for mathematical morphology with ImageJ

David Legland et al.

BIOINFORMATICS (2016)

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Article Biophysics

The use of zeta potential as a tool to study phase transitions in binary phosphatidylcholines mixtures

M. B. Sierra et al.

COLLOIDS AND SURFACES B-BIOINTERFACES (2016)

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Article Chemistry, Physical

Spreading of porous vesicles subjected to osmotic shocks: the role of aquaporins

Alice Berthaud et al.

SOFT MATTER (2016)

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Article Multidisciplinary Sciences

Posing for a picture: vesicle immobilization in agarose gel

Rafael B. Lira et al.

SCIENTIFIC REPORTS (2016)

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Article Multidisciplinary Sciences

SNARE-fusion mediated insertion of membrane proteins into native and artificial membranes

Gustav Nordlund et al.

NATURE COMMUNICATIONS (2014)

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Article Chemistry, Multidisciplinary

Defined DNA-Mediated Assemblies of Gene-Expressing Giant Unilamellar Vesicles

Maik Hadorn et al.

LANGMUIR (2013)

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Article Information Science & Library Science

Too Big to Fail: Large Samples and the p-Value Problem

Mingfeng Lin et al.

INFORMATION SYSTEMS RESEARCH (2013)

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Article Chemistry, Multidisciplinary

Cell-Free Protein Synthesis inside Giant Unilamellar Vesicles Analyzed by Flow Cytometry

Koji Nishimura et al.

LANGMUIR (2012)

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Article Multidisciplinary Sciences

Single Reconstituted Neuronal SNARE Complexes Zipper in Three Distinct Stages

Ying Gao et al.

SCIENCE (2012)

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Article Biophysics

Adhesion and Merging of Lipid Bilayers: A Method for Measuring the Free Energy of Adhesion and Hemifusion

Yen Sun et al.

BIOPHYSICAL JOURNAL (2011)

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Article Chemistry, Multidisciplinary

Complete Budding and Asymmetric Division of Primitive Model Cells To Produce Daughter Vesicles with Different Interior and Membrane Compositions

Meghan Andes-Koback et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

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Article Chemistry, Multidisciplinary

Programmed Vesicle Fusion Triggers Gene Expression

Filippo Caschera et al.

LANGMUIR (2011)

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Article Biophysics

Multi-Image Colocalization and Its Statistical Significance

Patrick A. Fletcher et al.

BIOPHYSICAL JOURNAL (2010)

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Article Biophysics

Direct Visualization of Large and Protein-Free Hemifusion Diaphragms

Joerg Nikolaus et al.

BIOPHYSICAL JOURNAL (2010)

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Article Chemistry, Physical

Determinants for membrane fusion induced by cholesterol-modified DNA zippers

Gudrun Stengel et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2008)

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Correction Chemistry, Multidisciplinary

DNA-induced programmable fusion of phospholipid vesicles (vol 129, pg 9584, 2007)

Gudrun Stengel et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)

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Review Cell Biology

Membranes of the world unite!

Leonid V. Chernomordik et al.

JOURNAL OF CELL BIOLOGY (2006)

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Review Biochemistry & Molecular Biology

The energetics of membrane fusion from binding, through hemifusion, pore formation, and pore enlargement

FS Cohen et al.

JOURNAL OF MEMBRANE BIOLOGY (2004)

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Article Multidisciplinary Sciences

On the relation between fluctuation and response in biological systems

K Sato et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

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Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis

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