Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFκB Signaling Pathway

Currently, there is a great demand for the development of nanomedicine aided wound tissue regeneration via silver doped nanoceuticals. Unfortunately, very little research is being carried out on antioxidants-doped silver nanometals and their interaction on the signaling axis during the bio-interface mechanism. In this study, c-phycocyanin primed silver nano hybrids (AgcPCNP) were prepared and analyzed for properties such as cytotoxicity, metal decay, nanoconjugate stability, size expansion, and antioxidant features. Fluctuations in the expression of marker genes during cell migration phenomena in in vitro wound healing scenarios were also validated. Studies revealed that physiologically relevant ionic solutions did not exhibit any adverse effects on the nanoconjugate stability. However, acidic, alkali, and ethanol solutions completely denatured the AgcPCNP conjugates. Signal transduction (RTPCR)-P-2 array demonstrated that genes associated with NF kappa B- and PI3K-pathways were significantly (p < 0.5%) altered between AgcPCNP and AgNP groups. Specific inhibitors of NF kappa B (Nfi) and PI3K (LY294002) pathways confirmed the involvement of NF kappa B signaling axes. In vitro wound healing assay demonstrated that NF kappa B pathway plays a prime role in the fibroblast cell migration. In conclusion, the present investigation revealed that surface functionalized AgcPCNP accelerated the fibroblast cell migration and can be further explored for wound healing biomedical applications.

Automated summary

There is a high demand for the development of nanomedicine for wound tissue regeneration with silver doped nanoceuticals. However, little research has been done on antioxidants-doped silver nanometals and their interaction during the bio-interface mechanism. This study prepared and analyzed c-phycocyanin primed silver nano hybrids (AgcPCNP) for their properties and their effects on gene expression and cell migration in wound healing scenarios. The results showed that AgcPCNP can accelerate fibroblast cell migration and has potential for wound healing biomedical applications.