期刊
JOURNAL OF THE NEUROLOGICAL SCIENCES
卷 367, 期 -, 页码 101-106出版社
ELSEVIER
DOI: 10.1016/j.jns.2016.05.054
关键词
Gliomas; Temozolomide resistance; Epithelial-to-mesenchymal transition; MGMT; Akt; beta-catenin
资金
- Key Clinical Specialty Discipline Construction Program
- National Natural Science Foundation of China [81472315, 81372692, 81302229]
Glioblastoma is one of the most lethal cancers in central nervous system, and some individual cells that cannot be isolated for surgical resection and also show treatment-resistance induce poor prognosis. Hence, in order to research these cells, we treated temozolomide (TMZ)-sensitive U87MG cells with 400 mu M TMZ in culture media for over 6 months and established TMZ-resistant cell line designated as U87/TR. We detected the MGMT status through pyrosequencing and western blotting, and we also assessed the proliferation, migration, EMT-like changes and possible activated signaling pathways in U87/TR cells. Our results demonstrated that U87/TR was MGMT negative, which indicated that MGMT made no contribution for TMZ-resistance of U87/TR. And U87/TR cells displayed cell cycle arrest, higher capacity for migration and EMT-like changes including both phenotype and characteristic proteins. We also revealed that both beta-catenin and the phosphorylation level of Akt and PRAS40 were increased in U87/TR, while we did not observe the phosphorylation of mTOR in U87/TR. It indicated that activation of Akt and Wnt/beta-catenin pathways may be response for the chemo-resistance and increased invasion of U87/TR cells, and the phosphorylation of PRAS40 and inactivated mTOR may be related to cell cycle arrest in U87/TR cells. (C) 2016 Elsevier B.V. All rights reserved.
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