期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/ijms24087191
关键词
Amomum tsaoko; constipation; gut microbiota; gut metabolites; gastrointestinal motility
Amomum tsaoko (AT) is a dietary botanical with laxative properties, and its active components and mechanisms are still unknown. The study found that the ethanol-soluble part of AT aqueous extract (ATES), particularly the total flavonoids (ATTF), promoted defecation in constipated mice by altering the gut microbiota and serotonergic synaptic pathway. ATTF increased the abundance of Lactobacillus and Bacillus while reducing Lachnospiraceae, and it also affected gut metabolites and the expression of genes involved in the serotonergic synaptic pathway. The study suggests that ATTF has the potential for the development of laxative drugs by regulating gut microbiota and serotonergic synaptic pathway.
Amomum tsaoko (AT) is a dietary botanical with laxative properties; however, the active ingredients and mechanisms are still unclear. The active fraction of AT aqueous extract (ATAE) for promoting defecation in slow transit constipation mice is the ethanol-soluble part (ATES). The total flavonoids of ATES (ATTF) were the main active component. ATTF significantly increased the abundance of Lactobacillus and Bacillus and reduced the dominant commensals, such as Lachnospiraceae, thereby changing the gut microbiota structure and composition. Meanwhile, ATTF changed the gut metabolites mainly enriched in pathways such as the serotonergic synapse. In addition, ATTF increased the serum serotonin (5-HT) content and mRNA expression of 5-hydroxytryptamine receptor 2A (5-HT2A), Phospholipase A2 (PLA2), and Cyclooxygenase-2 (COX2), which are involved in the serotonergic synaptic pathway. ATTF increased Transient receptor potential A1 (TRPA1), which promotes the release of 5-HT, and Myosin light chain 3(MLC3), which promotes smooth muscle motility. Notably, we established a network between gut microbiota, gut metabolites, and host parameters. The dominant gut microbiota Lactobacillus and Bacillus, prostaglandin J2 (PGJ2) and laxative phenotypes showed the most significant associations. The above results suggest that ATTF may relieve constipation by regulating the gut microbiota and serotonergic synaptic pathway and has great potential for laxative drug development in the future.
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