期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/ijms24054952
关键词
ciprofloxacin; lead; combined effects; behavior; biomarkers
Although the toxic effects of antibiotics and heavy metals have been studied extensively, their combined adverse impact on aquatic organisms is not well understood. This study assessed the acute effects of a ciprofloxacin and lead mixture on zebrafish behavior, enzyme activity, oxidative stress markers, and essential elements content. The results showed that exposure to the mixture impaired zebrafish behavior, altered essential elements content, and increased oxidative stress. This highlights the potential threat to living organisms from the simultaneous presence of antibiotics and heavy metals in the environment.
Even though the toxic effects of antibiotics and heavy metals have been extensively studied in the last decades, their combined adverse impact on aquatic organisms is poorly understood. Therefore, the objective of this study was to assess the acute effects of a ciprofloxacin (Cipro) and lead (Pb) mixture on the 3D swimming behavior, acetylcholinesterase (AChE) activity, lipid peroxidation level (MDA-malondialdehyde), activity of some oxidative stress markers (SOD-superoxide dismutase and GPx-glutathione peroxidase), and the essential elements content (Cu-copper, Zn-zinc, Fe-iron, Ca-calcium, Mg-magnesium, Na-sodium and K-potassium) in the body of zebrafish (Danio rerio). For this purpose, zebrafish were exposed to environmentally relevant concentrations of Cipro, Pb, and a mixture for 96 h. The results revealed that acute exposure to Pb alone and in mixture with Cipro impaired zebrafish exploratory behavior by decreasing swimming activity and elevating freezing duration. Moreover, significant deficiencies of Ca, K, Mg, and Na contents, as well as an excess of Zn level, were observed in fish tissues after exposure to the binary mixture. Likewise, the combined treatment with Pb and Cipro inhibited the activity of AChE and increased the GPx activity and MDA level. The mixture produced more damage in all studied endpoints, while Cipro had no significant effect. The findings highlight that the simultaneous presence of antibiotics and heavy metals in the environment can pose a threat to the health of living organisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据