期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/ijms24129782
关键词
heat stress; maize; leaf senescence; transcriptome sequencing
This study investigates the molecular mechanism of heat-stress-induced leaf senescence in maize seedlings. By screening different inbred lines, the researchers identified genes and pathways associated with heat stress response, reactive oxygen species, and photosynthesis. Furthermore, they found that silencing the ZmbHLH51 gene inhibits heat-stress-induced senescence in maize leaves. This research provides valuable insights into the understanding of heat stress and senescence in maize.
Maize, one of the world's major food crops, is facing the challenge of rising temperature. Leaf senescence is the most significant phenotypic change of maize under heat stress at the seedling stage, but the underlying molecular mechanism is still unknown. Here, we screened for three inbred lines (PH4CV, B73, and SH19B) that showed differentially senescing phenotypes under heat stress. Among them, PH4CV showed no obviously senescing phenotype under heat stress, while SH19B demonstrated a severely senescing phenotype, with B73 being between the two extremes. Subsequently, transcriptome sequencing showed that differentially expressed genes (DEGs) were generally enriched in response to heat stress, reactive oxygen species (ROS), and photosynthesis in the three inbred lines under heat treatment. Notably, ATP synthesis and oxidative phosphorylation pathway genes were only significantly enriched in SH19B. Then, the expression differences of oxidative phosphorylation pathways, antioxidant enzymes, and senescence-related genes in response to heat stress were analyzed in the three inbred lines. In addition, we demonstrated that silencing ZmbHLH51 by virus-induced gene silencing (VIGS) inhibits the heat-stress-induced senescence of maize leaves. This study helps to further elucidate the molecular mechanisms of heat-stress-induced leaf senescence at the seedling stage of maize.
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