4.7 Article

Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide

期刊

出版社

MDPI
DOI: 10.3390/ijms24076622

关键词

graphene oxide; inflammation; suture threads; inflammasome; polypropylene-GO composite

向作者/读者索取更多资源

This study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs) enriched with two different concentrations of graphene oxide (GO) in an in vitro model using primary human gingival fibroblasts (hGFs). The results showed that both concentrations of GO decreased the expression of inflammatory markers in hGFs treated with Escherichia coli lipopolysaccharide (LPS-E). The morphology and adhesion of hGFs on the PPSTBs-GO constructs were also examined.
Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties. The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NF?B p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E). The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis. Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E. The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR. Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NF?B p65/NLRP3 pathway.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据