期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/ijms24097999
关键词
lipid bilayer membrane; lipid raft; graphene oxide; fluorescence microscopy; atomic force microscopy; phase separation
The gel-phase domains preferentially distributed on graphene oxide (GO) flakes in a binary supported lipid bilayer, while the liquid crystalline phase increased on the bare SiO2 surface. The gel-phase domain was condensed more effectively on GO at the lower cooling rate.
The gel-phase domains in a binary supported lipid bilayer (SLB) comprising dioleoylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) were localized on graphene oxide (GO) deposited on a SiO2/Si substrate. We investigated the distribution of the gel-phase domains and the liquid crystalline (L-a) phase regions in DOPC+DPPC-SLB on thermally oxidized SiO2/Si substrates with GO flakes to understand the mechanism of the domain localization on GO. Fluorescence microscopy and atomic force microscopy revealed that the gel-phase domains preferably distributed on GO flakes, whereas the fraction of the L-a-phase increased on the bare SiO2 surface which was not covered with the GO flakes. The gel-phase domain was condensed on GO more effectively at the lower cooling rate. We propose that nucleation of the gel-phase domain preferentially occurred on GO, whose surface has amphiphilic property, during the gel-phase domain formation. The domains of the liquid ordered (L-o) phase were also condensed on GO in a ternary bilayer containing cholesterol that was phase-separated to the L-o phase and the liquid disordered phase. Rigid domains segregates on GO during their formation process, leaving fluid components to the surrounding region of GO.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据