Encapsulation of Lacto-N-biose based on ovalbumin and carboxymethyl cellulose microparticles: fabrication, characterisation and thermal stability

To improve the thermal stability Lacto-N-biose (LNB), it was encapsulated by the OVA-CMC microparticles system. When the mass ratio of OVA:CMC:LNB was 1.5:1:1, the particle size of microparticles was 623 +/- 4 nm, and the encapsulation efficiency and loading efficiency of 83 +/- 1.38% and 45 +/- 0.92% respectively. Analysis with the scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) revealed that the OVA-CMC-LNB microparticles were spherical and uniformly distributed. The hydrogen bonding and electrostatic interactions were the main forces to assemble the microparticles. The results of the thermogravimetric analysis showed that the structure of microparticles remained stable at 240 degrees C. The residual amount of LNB in microparticles reached 94.3 +/- 1.03% and 78.8 +/- 2.38% after being heated at 90 degrees C for 10 and 30 min, and the thermal stability was greatly improved. In vitro simulated digestion experiments showed that the release rate of LNB in microparticles reached 95 +/- 1.31%. These results provided a theoretical and technological approach for the construction of LNB delivery system improved thermal stability.

Automated summary

To enhance the thermal stability of Lacto-N-biose (LNB), it was encapsulated within OVA-CMC microparticles. With a mass ratio of OVA:CMC:LNB as 1.5:1:1, the microparticles exhibited a particle size of 623 +/- 4 nm, encapsulation efficiency of 83 +/- 1.38%, and loading efficiency of 45 +/- 0.92%. Characterization techniques such as SEM, FT-IR, and XRD confirmed the spherical and uniform distribution of the OVA-CMC-LNB microparticles. Hydrogen bonding and electrostatic interactions were identified as the main forces responsible for the microparticles' assembly. Thermal analysis revealed that the microparticles remained structurally stable at 240 degrees C, greatly improving the thermal stability of LNB. In vitro digestion experiments demonstrated a release rate of LNB from the microparticles of 95 +/- 1.31%. These findings provide a theoretical and technological framework for enhancing the thermal stability of LNB delivery systems.