4.7 Article

Emergence of lnu(C) variant conferring lincomycin resistance in Campylobacter coli of chicken origin

期刊

出版社

ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2023.110098

关键词

Food-borne pathogen; Campylobacter; Lnu(C); MTnSag1-like transposon

向作者/读者索取更多资源

Lincomycin is frequently used in veterinary medicine and food animal production for treating respiratory and gastrointestinal infections. This study identified a lnu(C) variant in Campylobacter from chickens that confers resistance to lincomycin. The lnu(C) variant showed amino acid substitutions compared to the originally reported lnu(C) from Streptococcus agalactiae. Cloning the lnu(C) variant into lincomycin-susceptible Campylobacter confirmed its function in increasing resistance.
Lincomycin is widely used in respiratory and gastrointestinal infection in veterinary medicine and food animal production. Campylobacter members are vital foodborne pathogens causing campylobacteriosis, and the resistance to lincosamides is seldom reported. To date, only the rRNA methyltransferase Erm(B) has been confirmed to be associated with lincosamides resistance in Campylobacter. In this study, we identified a lnu(C) variant conferring lincomycin resistance in this pathogen of chicken origin. The Lnu(C) encoded by this gene variant showed substitution at position 8 (Asn8Lys), 11 (Phe11Leu) and 112 (Leu112Phe), when compared with the firstly reported Lnu(C) from Streptococcus agalactiae. Cloning of the lnu(C) variant into lincosamide-susceptible Campylobacter jejuni NCTC 11168 confirmed its function in conferring resistance to lincomycin with the 32-fold increased MICs. Sequencing analysis showed that the lnu(C) variant was located within a MTnSag1-like transposon together with insLNU, which is inserted between panB and cj0299 genes on the chromosome. lnu(C) gene was distributed among C. coli globally, and various STs were involved in the dissemination of lnu(C). Although transposition mediated by MTnSag1-like transposon failed to occur, the horizontal transfer mediated by natural transformation and reservoir for resistance genes may facilitate their adaptation to the antimicrobial selection pressure in chickens, which should not be ignored.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据