期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 232, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.12.263
关键词
Protein fibrillation; Gold nanoparticles; Antioxidant activity; Cytocompatibility; Alzheimer's disease; Hen egg white lysozyme
The research found that biogenic gold nanoparticles can inhibit protein amyloidogenesis, which is linked to chronic diseases such as Alzheimer's and Parkinson's disease. The synthesized nanoparticles, stabilized with quercetin-functionalized tara gum, demonstrated strong anti-aggregation and fibrillation effects against amyloidogenic proteins. They also showed potential for countering protein aggregation and associated disease conditions.
Aberrant protein misfolding and/or aggregation and fibrillation has been linked to the pathogenesis of several debilitating chronic diseases including Alzheimer's and Parkinson's disease. Inhibiting protein amyloidogenesis has been proposed as a viable strategy to prevent or ameliorate associated disorders. Herein, we investigated the anti-amyloidogenic properties of biogenic gold nanoparticles (QTG-GNP) prepared via a simple green chemistry route and stabilized by quercetin-functionalized tara gum (QTG). The synthesized QTG-GNP was extensively characterized for its physicochemical attributes via UV-visible spectroscopy, TEM, FESEM, EDX, DLS/Zeta potential, FTIR, RAMAN, XRD, XPS, and TGA analyses, as well as for its biological properties. The results revealed that small-sized (5.01 +/- 1.17 nm), well-dispersed, highly stable and round-shaped biogenic gold nanoparticles were successfully synthesized at room temperature with QTG as the sole reductant /stabilizer. Importantly, QTG-GNP demonstrated potent anti-aggregation and fibrillation inhibitory effects against amyloidogenic hen egg white lysozyme (HEWL). Also, QTG-GNP was able to dissociate pre-formed HEWL amyloid fibrils. Furthermore, the constructed nanoparticles exhibited potent anti-radical activities against DPPH center dot and ABTS center dot(+) and were cytocompatible with mouse L929 fibroblast cells. On the basis of these findings, it was established that QTG-GNP holds strong prospects for further development as an agent for countering protein aggregation and associated disease conditions.
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