期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 238, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2023.124078
关键词
Carbon quantum dots; ?-Tricalcium phosphate; Chitosan; Silk fibroin; Cytotoxicity; Wound healing
In this study, a nanofiber wound dressing composed of chitosan/silk fibroin/N-doped carbon quantum dots/alpha-tricalcium phosphate was synthesized and characterized. The results showed that the nanofiber exhibited excellent biocompatibility and antimicrobial properties, promoting wound healing.
A highly porous nanofibrous network that can functionalize antibacterial and therapeutic agents can be considered a suitable option for skin wound healing. In this study, alpha-tricalcium phosphate (alpha-TCP)/nitrogen-doped carbon quantum dots (N-CQDs) nanocomposite was synthesized and then applied to the fabrication of novel chitosan (CS)/silk fibroin (SF)/N-CQDs/alpha-TCP wound dressing via electrospinning system. The prepared nanomaterials were well characterized using X-ray diffraction, Fourier-transform infrared, scanning and trans-mission electron microscopes analyses, and antibacterial assay. Furthermore, nanofibers were evaluated regarding their physical properties, such as tensile behavior, water uptake capacity, and water contact angle. The results reveal that CS/SF/N-CQDs/alpha-TCP showed lower MIC values against E. coli and S. aureus (1.45 +/- 0.26 mg/ mL and 1.59 +/- 0.12 mg/mL) compared to other synthesized materials. Also, in-vitro investigations were per-formed, and the MTT assay on the HFF cell line revealed that CS/SF/N-CQDs/alpha-TCP nanofiber could possess good biocompatibility. Interestingly, the scratch test proved that faster cell migration and proliferation occurred in the presence of CS/SF/N-CQDs/alpha-TCP 73.23 +/- 2.71 %). Finally, we examined the wound healing ability of CS/SF/N-CQDs/alpha-TCP nanofiber using an animal model. The results confirmed that produced nanofiber could efficiently promote wound closure by 96.73 +/- 1.25 % in 12 days. Histopathological analyses verified accelerated re-epithelization and well-structured epidermis in CS/SF/N-CQDs/alpha-TCP nanofiber-treated group. Based on our findings, the CS/SF/N-CQDs/alpha-TCP nanofiber with excellent antimicrobial properties is highly suitable for wound healing and skin tissue regeneration applications.
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