The preventive effect of Glycyrrhiza polysaccharide on lipopolysaccharide-induced acute colitis in mice by modulating gut microbial communities

Acute colitis is characterised by an unpredictable onset and causes intestinal flora imbalance together with microbial migration, which leads to complex parenteral diseases. Dexamethasone, a classic drug, has side effects, so it is necessary to use natural products without side effects to prevent enteritis. Glycyrrhiza polysaccharide (GPS) is an alpha-d-pyranoid polysaccharide with anti-inflammatory effects; however, its anti-inflammatory mech-anism in the colon remains unknown. This study investigated whether GPS reduces the lipopolysaccharide (LPS)-induced inflammatory response in acute colitis. The results revealed that GPS attenuated the upregulation of tumour necrosis factor-alpha, interleukin (IL)-1 beta, and IL-6 in the serum and colon tissues and significantly reduced the malondialdehyde content in colon tissues. In addition, the 400 mg/kg GPS group showed higher relative expressions of occludin, claudin-1, and zona occludens-1 in colon tissues and lower concentrations of diamine oxidase, D-lactate, and endotoxin in the serum than the LPS group did, indicating that GPS improved the physical and chemical barrier functions of colon tissues. GPS increased the abundance of beneficial bacteria, such as Lactobacillus, Bacteroides, and Akkermansia, whereas pathogenic bacteria, such as Oscillospira and Ruminococcus were inhibited. Our findings indicate that GPS can effectively prevent LPS-induced acute colitis and exert beneficial effects on the intestinal health.

Automated summary

This study investigated the anti-inflammatory effects and mechanism of action of glycyrrhiza polysaccharide (GPS) in acute colitis. The results showed that GPS attenuated the upregulation of pro-inflammatory cytokines and oxidative stress markers in colon tissues, improved the physical and chemical barrier functions of colon tissues, and modulated the gut microbiota composition. These findings suggest that GPS can effectively prevent acute colitis induced by lipopolysaccharide and exert beneficial effects on intestinal health.