Acylated pectin/gelatin-based films incorporated with alkylated starch crystals: Characterization, antioxidant and antibacterial activities, and coating preservation effects on golden pomfret

Pectin and starch crystals were modified by ethyl gallate and octadecyl-trimethoxysilane, respectively, followed by using acylated pectin (AP) and alkylated starch crystals (ASCs) as bioactive reagents and hydrophobic enhancers to improve the physiochemical properties of gelatin-based films and evaluate their coating preservation effects on golden pomfret. The properties of AP and ASC were investigated by Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-vis), proton-nuclear magnetic resonance (1H NMR) and X-ray diffraction (XRD). The ethyl-gallate-modified pectin/gelatin (AP/G) containing 3 % ASC (AP/G/ASC-3 %) was shown to have the maximum tensile strength and Young's modulus of all the tested composite films. The AP/G containing 10 % ASC exhibited a water contact angle higher than 94 degrees, coupled with a significant improvement in UV-shielding efficiency. FTIR and SEM analysis of the AP/G/ASC-3 % film indicated that the molecular interactions in the composite film components were noncovalent linkages, including hydrogen bonds, hydrophobic interactions, and electrostatic interactions, contributing to homogeneous and smooth microstructures. Additionally, the solutions of AP/G and AP/G/ASC composite films presented obvious antioxidant and antibacterial activities against Escherichia coli and Staphylococcus aureus. Furthermore, the AP/G and AP/G/ASC active coatings could effectively inhibit lipid oxidation and improve the textural acceptability of golden pomfret (Trachinotus blochii) fillets during 4 degrees C storage.

Automated summary

Pectin and starch crystals were modified with ethyl gallate and octadecyl-trimethoxysilane, respectively, to improve the properties of gelatin-based films. Acylated pectin and alkylated starch crystals were used as bioactive reagents and hydrophobic enhancers. The resulting composite films exhibited enhanced physical properties and showed antioxidant and antibacterial activities. Additionally, these active coatings effectively inhibited lipid oxidation and improved the texture of golden pomfret fillets during storage.