期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 241, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2023.124504
关键词
Schizophyllan; Schizophyllum commune; Fermentation optimization; Antioxidant activity; Anti-photoaging; Melanin
In this study, SPG-producing strains of S. commune from different provinces in China were screened. A candidate strain (NTU-1) with a high SPG yield was chosen, and the fermentation conditions were optimized. The highest reported SPG concentration of 21.3 g/L was obtained in a 5-L bioreactor with a reduced fermentation cycle of five days.
Schizophyllan (SPG), a /3-glucan produced by the fungus Schizophyllum commune, possesses a /3-(1-+ 3)-linked backbone with single /3-(1-+ 6)-linked glucose side chains at approximately every third residue. In this study, we screened SPG-producing strains of S. commune from different provinces in China. A candidate strain (NTU-1) with a high SPG yield was chosen, and the fermentation conditions were optimized. The optimal carbon and nitrogen sources were sucrose (40 g/L) and yeast extract (20 g/L), respectively. The optimal conditions for pH and temperature were 5.0 and 28 degrees C, respectively. Inclusion of 0.2 mg/L of 2,4-Dichlorophenoxyacetic acid in the medium further increased the SPG concentration. In a 5-L bioreactor, the fermentation cycle was reduced from the initial seven days to five days, and the concentration of SPG obtained was 21.3 g/L, which is the highest reported to date. In addition, we evaluated the bioactivity of the SPG prepared using strain NTU-1. The results showed that SPG had certain characteristics of anti-oxidation, anti-photoaging, and inhibition of melanin pro-duction, making it a promising reagent for skin care.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据