期刊
HOLZFORSCHUNG
卷 77, 期 4, 页码 230-239出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/hf-2022-0141
关键词
(CO2)-C-13; Ginkgo biloba L; lignin; quantitative NMR; solid-state NMR
This study cultivated Ginkgo biloba L. saplings in a growth chamber with C-13 for two months. The C-13 ratio of the newly developed xylem region was evaluated using high lateral resolution secondary ion mass spectrometry and thioacidolysis/GC-MS. Solid-state C-13 direct polarisation/magic angle spinning (DP/MAS) NMR measurements were conducted for different wood samples, and the results suggested structural alterations of lignin during the sample preparation procedure, including ball milling.
Ginkgo biloba L. saplings were cultivated in an airtight growth chamber with (CO2)-C-13 for two months. The C-13 ratio of the newly developed xylem region was ca. 85%, evaluated by high lateral resolution secondary ion mass spectrometry and thioacidolysis/GC-MS. Quantitative solid-state C-13 direct polarisation/magic angle spinning (DP/MAS) NMR measurements with high-speed MAS of 70.0 kHz were conducted for cutting-milled wood (CMW), ball-milled wood (BMW), and enzymatically saccharified lignin (EL) samples. In addition, quantitative liquid-state C-13 NMR measurements were carried out for EL in DMSO-d(6). Major lignin aromatic signals were classified into three groups of aromatic carbons of C-H, C-C, and C-O, and their area ratio was compared within these measurements. EL samples in solid- and liquid-state showed nearly the same results. However, the results for CMW and BMW in solid-state NMR suggest the structural alteration of lignin within the sample preparation procedure, including ball milling.
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