Vascular permeability in HPV plus oropharyngeal cancers aids in fluorescent image-guided transoral robotic surgery using indocyanine green

BackgroundIndocyanine green (ICG) fluorescent image (FI)-guided surgery has demonstrated success in improving intraoperative visualization and tumor resections. The objectives were to evaluate the use of IGC in FI-guided transoral robotic surgery (TORS) and the underlying molecular mechanism. MethodsHPV+ oropharyngeal squamous cell carcinoma (OPSCCa) patient (n = 10) undergoing TORS were enrolled in this prospective study. Participants received intravenous ICG. Excised tissues were evaluated for ICG accumulation, tumor demarcation, and pathological characteristics using In-vivo imaging system (IVIS), histology, and RNA sequencing. ResultsICG accumulation was significantly increased in primary tumor and pathological lymph nodes compared with normal tissues (p < 0.001). IVIS was 91.3% accurate in identifying OPSCCa in excised tissues; the correlation between IVIS- and histologically determined tumor tissues was significant (R-2 = 0.8301; p = 0.001). Genes associated with vascular and angiogenic signaling pathways were significantly upregulated in OPSCCa tissues. ConclusionICG effectively demarcates tumor margins in OPSCCa, due to the increased upregulation of genes associated with vascular permeability.

Automated summary

This study evaluated the use of indocyanine green (ICG) in FI-guided transoral robotic surgery (TORS) and its underlying molecular mechanism. The results showed that ICG accumulation increased significantly in primary tumors and pathological lymph nodes. IVIS accurately identified oropharyngeal squamous cell carcinoma (OPSCCa) in excised tissues, and genes associated with vascular and angiogenic signaling pathways were upregulated in OPSCCa tissues.