期刊
GENOME BIOLOGY AND EVOLUTION
卷 15, 期 6, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evad090
关键词
satellite; major ribosomal RNA genes; mobile elements; Lepidoptera
This study investigates the peculiar organization of rDNA in Lepidoptera and suggests that satellite arrays facilitate the homology-mediated spread of rDNA through the integration of extrachromosomal rDNA circles or ectopic recombination.
Genes for major ribosomal RNAs (rDNA) are present in multiple copies mainly organized in tandem arrays. The number and position of rDNA loci can change dynamically and their repatterning is presumably driven by other repetitive sequences. We explored a peculiar rDNA organization in several representatives of Lepidoptera with either extremely large or numerous rDNA clusters. We combined molecular cytogenetics with analyses of second- and third-generation sequencing data to show that rDNA spreads as a transcription unit and reveal association between rDNA and various repeats. Furthermore, we performed comparative long read analyses among the species with derived rDNA distribution and moths with a single rDNA locus, which is considered ancestral. Our results suggest that satellite arrays, rather than mobile elements, facilitate homology-mediated spread of rDNA via either integration of extrachromosomal rDNA circles or ectopic recombination. The latter arguably better explains preferential spread of rDNA into terminal regions of lepidopteran chromosomes as efficiency of ectopic recombination depends on the proximity of homologous sequences to telomeres.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据