期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 335, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2023.114233
关键词
Insulin; IGF; Periplaneta; Pars intercerebralis; Corpus cardiacum
Among the nine genes related to insulin and insulin-like growth factor in the American cockroach, seven are expressed in the central nervous system. The cells in the pars intercerebralis show significant expression of five of these genes. Antisera testing confirms that these cells are neuroendocrine and project to the corpora cardiaca. In contrast to fruit flies, multiple cell types in cockroaches express different genes, indicating a more complex regulation by insulin and IGF-related peptides in cockroaches.
Of the nine genes of the American cockroach, Periplaneta americana, coding for peptides related to insulin and insulin-like growth factor, seven show significant expression in the central nervous system as demonstrated by the polymerase chain reaction on reverse transcribed RNA. In situ hybridisation shows that five of those are expressed by cells in the pars intercerebralis. Antisera raised to the predicted peptides show that these cells are neuroendocrine in nature and project to the corpora cardiaca. Interestingly, there are at least three cell types that each express different genes. This contrasts with Drosophila where a single cell type expresses a number of genes expressing several such peptides. Whereas in Drosophila the neuroendocrine cells producing insulin-like peptides also express sulfakinins, the arthropod orthologs of gastrin and cholecystokinin, in Periplaneta the sulfakinins are produced by different cells. Other neuropeptides known to be produced by the pars intercerebralis in Periplaneta and other insect species, such as the CRF-like diuretic hormone, neuroparsin, leucokinin or myosuppressin, neither colocalize with an insulin-related peptide. The separate cellular localization of these peptides and the existence of multiple insulin receptors in this species implies a more complex regulation by insulin and IGF-related peptides in cockroaches than in the fruit fly.
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